The wound healing assay, commonly known as the scratch assay, is a standard in vitro technique used to study collective cell migration and quantify the effects of various treatments or conditions on cell motility. This protocol provides a step-by-step guide for performing a reproducible wound healing assay using common laboratory equipment.

Principle of the Wound Healing Assay This method relies on creating a defined, cell-free gap ("wound" or "scratch") within a confluent monolayer of cultured cells. The rate at which cells migrate from the edges to close this gap is monitored over time, typically using time-lapse microscopy. Comparing the closure rates between different experimental conditions allows researchers to assess factors influencing cell migration.

Read our full step-by-step scratch assay SOP at: https://www.clyte.tech/post/how-to-do-wound-healing-scratch-...

Learn more about how CellCut can elevate your scratch assay: https://www.clyte.tech/post/a-high-throughput-device-for-con... Explore more of our detailed SOPs: https://www.clyte.tech/news/categories/sop