I believe that Eukaryotes then from Archae.
For example, suppose horizontal gene transfer occurs from organism X to organism Y. Does that mean Y is now a branch of X?
* Does it depend on how much was transferred?
* Does it matter only if the specific sequence was passed down? If so, how much mutation is too much mutation?
* What if the same end-result occurred through a retrovirus instead of a plasmid. Is the virus an ancestor too?
* What if the swap was simultaneous and bidirectional?
* What about transitive links to organisms W, V, U that did the same?
* Are mitochondria "us" yet? If so, are we the ancestors if they redevelop enough machinery to "escape"?
etc.
Chance-Device•7h ago
It’s one quarter of an image flipped horizontally and then vertically, you can see the patterns.
It’s a bit odd to do that? Shouldn’t it just be the original EM image?
jiggawatts•7h ago
Publish or perish needs to end.
RicoElectrico•7h ago
observationist•6h ago
After a bit of digging - it looks like it's done to sharpen features as one of the standard steps in producing these images. Where there are rotational symmetries in the things they're looking at, they focus on the smallest unit, and then rotate accordingly. If you had a trilateral symmetry, or hexagonal structure, they'd rotate 3 or 6 times around the center.
You're not getting a real image of the thing, but apparently it's got data from those other segments mixed in with the rotations, so you're getting a kind of idealized structure, to make the details being studied pop out, but if you have some sort of significant deviation, damage, or non symmetric feature it'll show up as well.
It's called "imposed symmetry" https://discuss.cryosparc.com/t/what-is-actually-occuring-wh...
Neat stuff, cool thing to catch!
Terr_•3h ago
Bjartr•6h ago
A different paper, this figure shows a number of cryo-em images, including a simulation, and they all show the same degree of pattern symmetry https://www.researchgate.net/figure/Central-sections-through...
First figure in this third paper also shows symmetry of small patterns https://journals.asm.org/doi/10.1128/jvi.00990-22
Chance-Device•6h ago
I still think it’s super weird that it looks exactly like an EM image, but is generated. Anyway, good to know!