From what I’ve gathered the actual splicing is partly automated today and relatively straightforward if somewhat tedious. The big variable is the context. New construction should have relatively few variables.

With repair, everything goes out the window. I just talked to him last night and he was out on a cable cut repair all night Friday. Middle of a snowstorm, maps were not accurate, repair site was very difficult to work in.

For example, see Table 1: https://book.bionumbers.org/how-quickly-do-different-cells-i...

You /could/ compute a global mean or median mitosis rate, and show how it changes/doesn't change with age, but it wouldn't say very much biologically. A narrower analysis that considers cell type and other context could be meaningful.

https://en.wikipedia.org/wiki/Helicase

The wild thing is that it doesn't have a 'gas tank' of ATP to drive the reaction, it goes this fast while being fueled one molecule at a time from the environment.

Where does the ATP come from?

Buckle up my mechanical engineer friend - https://www.youtube.com/watch?v=OT5AXGS1aL8

I've watched that video a hundred times and it still gives me chills haha.

Also you may be interested in flagellar motors: https://www.youtube.com/watch?v=VPSm9gJkPxU

I can see how a cell is far too complicated to contemplate at this time. But, if focusing on the video of the DNA replication complex. (DNA strand + a few enzymes), I wonder if it could be in the realm of doable within the coming years or decades.

Re water... yea... I suspect explicit solvents are the way to go. So, you are not just simulating the protein and DNA molecules, but also each water.