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Blood test detecting Long Covid in kids with 94% accuracy microclots

https://www.researchsquare.com/article/rs-7483367/v1
81•thenerdhead•2h ago

Comments

kace91•2h ago
Can someone knowledgeable explain the current understanding of Covid’s long time effects? I thought it was still a big unknown and long COVID was still debated as to even having a clear definition.
dmd•2h ago
If only there were some sort of link here with that sort of information!
kace91•1h ago
I did read the article. It only talks about detecting long covid in children, which is different to understanding its effect on adults and the long terms effect of COVID in general (vs long COVID, which if understand correctly it’s a different syndrome to just “ long term expected outcomes from having had COVID”).
PaulKeeble•5m ago
The WHO [1] has a clinical definition of the disease and some basic data on chance of Covid infections turning into Long Covid. Then the meta study by Eric Topol and Zayad Ali-al from last year is probably a good primer into what is known about the disease that is well established science [2].

Its not a big unknown anymore, its very prevalent, it has a lot of symptoms, it has a clinical definition but its problematic and there are many diagnostic tests that can detect parts of the condition but none has yet reached sufficient prominence to be adopted by healthcare. Healthcare is largely ignoring that Long Covid exists so you can't get diagnosed with it but its very much a real thing and a lot of people have it.

[1]https://www.who.int/news-room/fact-sheets/detail/post-covid-...

[2] https://erictopol.substack.com/p/long-covid-at-3-years

diffeomorphism•2h ago
94% accuracy sounds extremely bad, no?

https://www.ssph-journal.org/journals/public-health-reviews/...

> Prevalence estimated (...) 2%–3.5% in primarily non-hospitalized children.

So a fake test always saying "No" would be more accurate at 96.5% accuracy.

mouse_•2h ago
Sample size of 59 also seems worse than useless; I'm no researcher so maybe there's something I'm missing here but, doesn't seem very good.

Junk science?

mapontosevenths•1h ago
It's just an early study, not junk.

The primary conclusion of this research was basically just "this looks like it would be worth doing more research on." Which is a fair conclusion for a study this small.

resoluteteeth•1h ago
If you read the actual link I don't think they're saying that using it as a covid test with some specific threshold of microclots has a 94% accuracy but just that the raw microclot count has a 94% accuracy.

The title on hn which implies that seems to be inaccurate and it's not the original title of the article.

diffeomorphism•1h ago
No, that does not seem to be what they are saying.

> We evaluated the diagnostic power of the device in a cohort of 45 LC patients and 14 healthy pediatric donors. We estimated a 94% accuracy for the microclot count using the devices, significantly higher than the traditional counting of microclots on slides (66% accuracy).

They are comparing the predictive power and using accuracy (instead of sensitivity, recall, F1, etc.). For their method "using the devices", they compute an accuracy of the predictive power, not of the count, of 94%. For the previous method they say the accuracy is 66%.

Basic questions: Is accuracy even a good metric for this? Is 94% a good value or just the difference between bad and very bad?

It might very well be that their improvement is from bad to really good, but the point is that a raw stat of "94% accuracy" is useless without context and so is the headline.

Zak•1h ago
The sensitivity of such a test would be 0. This test had a sensitivity of 91% versus 61% for the glass slide count method, which is a large improvement.

The sample size is pretty small here and the control group even smaller. The paper concludes that a larger study is necessary to confirm the result.

diffeomorphism•1h ago
That is exactly why I gave that example. Why does the headline focus on accuracy then?
porridgeraisin•54m ago
To get you to click on it :-)
shawabawa3•1h ago
Accuracy is a nonsense word in this context

Tests have a sensitivity (1 - percentage of false negatives) and specificity (1 - percentage of false positives)

"Accuracy" usually refers to sensitivity. If specificity is near 100% and the test is cheap/fast even low sensitivity can be good

On the other hand you could have sensitivity of 100% but the test could be useless if specificity is low and the condition is rare

diffeomorphism•54m ago
No, it is a well defined term in this context and does not refer to sensitivity.

https://pmc.ncbi.nlm.nih.gov/articles/PMC4614595/#:~:text=Ac...

That is exactly why I gave the trivial example of an "always No" test. It has perfect specificity (zero false positives) and has accuracy corresponding to prevalence. The sensitivity is zero, however, which is the point.

Wintamute•2h ago
Can they disambiguate between spike protein microclots produced by natural infection vs. those generated by the vaccines.
notrealyme123•1h ago
I am curious about the working hypothesis leading to this question.
zacharachnia•1h ago
Hypothesis: microclots are caused by the viral spike protein. The mRNA vaccine produces the viral spike protein. Therefore, a non-zero percentage of microclots are caused by spike protein originating from the vaccine.
DaSHacka•1h ago
One of the working theories on people suffering long COVID-like symptoms after receiving an mRNA COVID vaccine may apparently be related to spike proteins found in the patient's blood far longer than is typical/intended. This is apparently similar to long COVID patients.

At least, that's my layman's understanding when I was following it some years ago. I'm not sure if there's been more recent studies that have found more concrete links since then, but I suspect GP is in the same boat, which is why they asked.

tim333•29m ago
I've looked up quite a lot of research about vaccines doing damage and the usual result is yes they do but it's about 100x less bad than covid itself.
Tarsul•2h ago
This is (probably) not a Long Covid story but I found that bloodletting (for which I even received money!) gave me back the energy that I was missing for the last few years (e.g. it was impossible to build stamina). I also read about a study about the positive effects of bloodletting[1] that somehow is not all the rage in mainstream news, which I find perplexing. If it might be so easy to improve your health (for some of us), why isn't this discussed or studied more broadly?

[1]https://www.sciencedaily.com/releases/2012/05/120529211645.h...

"the patients who gave blood had a significant reduction in systolic blood pressure (from 148 mmHg to 130 mmHg) as well as reduction in blood glucose levels and heart rate, and an improvement in cholesterol levels (LDL/HDL ratio)."

spockz•1h ago
The lower blood pressure I can explain by having a lower volume of blood. But the glucose and cholesterol have to go somewhere. Where do they go? Are they filtered in the process after blood letting?
mort96•1h ago
If it's in the blood, doesn't it just leave the body together with the blood?
aesh2Xa1•1h ago
Yes, but the relative amount per volume blood should be the same. I think that's why he's asking.

If so, the answer is that the body replenishes plasma in a day and red cells in six weeks (redcrossblood.org FAQ). The relative amount does change quickly.

mkoubaa•1h ago
If this were the case you could do a natural study on this by comparing menstruating women with the rest of the population
jtbayly•1h ago
This has been done. Women seem to have certain health benefits that stop after menopause. Reading about it was the first time I wondered whether blood letting made sense.
Thorrez•1h ago
When people donate blood, on average they donate about 10x as much as a woman typically loses during menstruation.
aesh2Xa1•1h ago
I looked up the amount of blood lost due to a menstruation cycle, and the answer is around 50 ml.

OP's linked paper has "the iron-reduction patients had 300ml of blood removed at the start of the trial and between 250 and 500ml removed four weeks later."

A blood donation removes 500 ml, so about a year of menstruation all at once. You can donate every two months, besides.

So, yes, if there is an effect then we might expect the magnitude of the effect to differ. Or else we'd expect a paper cut to also have the same effect.

Sex biological difference could matter as well.

jfengel•59m ago
Menstruating women don't lose nearly that much blood.

Even if they did, the hormonal effects would likely swamp anything else. Which is a huge problem: women are routinely excluded from studies to avoid that, meaning we have no idea what the effects are on women.

jeremy151•1h ago
I live in an area with PFAS contaminated ground water (which I now aggressively filter.) To me giving blood just kind of makes sense, if there is a class of things that can enter your blood and never leave, and does not replicate on its own, why not perform a regular "oil change" and hopefully help some people at the same time. Some study has been done:

https://pmc.ncbi.nlm.nih.gov/articles/PMC8994130/

The study specifically does not look at the effect on recipients, though the donation centers do not disallow such donations. My presumption is that the donation is a net positive all around. If study comes to show the contrary, I'll certainly revise my approach.

TaupeRanger•12m ago
“I’ll do something which might be beneficial or harmful to me (I don’t know) and if given evidence of harm (likely never) I’ll stop doing it.” Ok…have fun I guess.
clanky•38m ago
> I also read about a study about the positive effects of bloodletting[1] that somehow is not all the rage in mainstream news, which I find perplexing. If it might be so easy to improve your health (for some of us), why isn't this discussed or studied more broadly?

If this works, how is anyone going to make money off of it?

comboy•36m ago
> If it might be so easy to improve your health (for some of us), why isn't this discussed or studied more broadly?

I think you just need to reach for a literature that's a few hundred years older maybe.

tim333•22m ago
That's interesting - I've had low energy and somewhat raised blood pressure since covid. Maybe I'll give it a go.
withinboredom•14m ago
I assume we are built to lose some blood. I imagine throughout most of our species development, injuries were somewhat common on a regular basis. Just looking at the scars on my own body, from a quite active childhood and young adult period outdoors, I am extrapolating. n = 1
itsme0000•6m ago
Yeah sounds like you get your medical advice from the Kremlin. There no conspiracy to hide the medical benefits of leaching, can you imagine if that actually worked? Every doctor in the world would have to be a complete moron not to notice.
tgsovlerkhgsel•1h ago
The paper claims that 1/5 of people experience Long COVID after an infection. Given that approximately everyone has caught COVID by now, this does not track with how rarely I've heard of people with it.

Wikipedia lists much lower numbers on https://en.wikipedia.org/wiki/Long_COVID (6–7% in adults, ~1% in children, less after vaccination.) and seems to use a more liberal definition than this paper, as it mentions "Most people with symptoms at 4 weeks recover by 12 weeks" (while the paper only considers it "long COIVD" if symptoms last past 3 months).

I've found studies (peer reviewed, as far as I can tell) claiming anything from well under 10% to well over 30%.

What's going on here?

victorbjorklund•1h ago
Could it be that you can have different severity of long covid? Someone with very severe symptoms will notice it but someone with small symptoms might not realise it (and instead think they are just less fit)
Thorrez•1h ago
>after an acute SARS-CoV-2 infection.

Maybe not all infections are considered "acute".

DrewADesign•1h ago
Acute, in this context, just means the infection had a finite (and usually relativity short) duration. The opposite is a chronic condition, which is what “long covid” would be in this case.
cheschire•1h ago
A lot of symptoms of long covid mirror the symptoms of unhealthy living, which may make it so people either dismiss their own symptoms and don't seek diagnosis, or their complaints are dismissed by others for needing to simply needing to improve externalities.
MontyCarloHall•9m ago
Couldn’t it be the other way round, that changes in health caused by other external factors erroneously get blamed on COVID?

For instance, a disproportionate amount of long COVID cases are reported by women between the ages of 40 and 60, the exact age range when most women experience menopause [0]. Menopause can cause brain fog, fatigue, and other symptoms that mirror those of long COVID. Since pretty much everyone has had COVID, it’s a basic statistical certainty that many women caught COVID exactly when their menopausal symptoms started (whose onset can be extremely sudden), and falsely causally associate the two. The exact same conflation likely happens in children, who also go through several profound developmental shifts.

[0] https://telegraph.co.uk/news/2022/12/28/long-covid-may-actua...

Ozzie_osman•1h ago
The paper says "One in five children." I wouldn't be surprised at all. Children are very dynamic, changing often as they grow and go through different developmental stages (which may include periods where they seem more tired or more cranky, etc) . They also often lack the language and agency to explain what's going on with their bodies.

I don't see how you'd know the exact number without a solid diagnostic check.

tiahura•1h ago
I'm an ambulance chaser. I look at medical records all day. I've never seen anyone complaining of long covid.
rr808•57m ago
I have a 87 year old uncle who says he has long covid because he gets tired and needs a nap in the afternoon. I'm half the age and felt the same even before 2020.
Llamamoe•54m ago
It takes very severe disability for it not to be brushed off as depression, anxiety, burnout, or blamed on lifestyle.

Additionally, a lot of those numbers are based on earlier strains of COVID, which were much more severe.

I suspect the 1/5 figure is largely true for "has some degree of cardiovascular damage and worsened general health after COVID", but the number of people actually disabled by the condition is much lower.

That said, any loss of ability is a sad thing, and I am incredibly disappointed that we did not introduce any shared indoor space air quality legislation post-pandemic.

notmyjob•46m ago
And the lipid nanoparticle based Covid prevention shots caused myocarditis in some younger males, so earlier incidence of cardiovascular complications vs. the present (when most people especially younger healthy males are choosing not to take updated boosters) would correlate with that as well.
clanky•42m ago
The one Thai prospective study that I saw on this subject (the only one I ever found which measured biomarkers of heart damage like troponin before and after injection, which is utterly shameful), estimated a rate of heart damage in adolescent males in the range of 1-3%. So even that would be nowhere near enough to reach the 1/5 number.
wahnfrieden•38m ago
The legislation and policy changes being introduced is to remove funding and access to vaccines. Of course we will not legislate for clean air.
clanky•48m ago
The numbers in the range of 1/5 usually include in the definition things like "a cough that lasts six weeks after the acute phase of infection and then goes away," which is not what most people think of when they hear the term Long COVID (and is not even unique to SARS-CoV-2).

For a variety of reasons, hyping the threat of infection has been a pretty widespread practice among the medical and scientific community since COVID began. There's no way on earth 1 out of 5 kids are still experiencing symptoms 3 months out.

djtango•47m ago
After covid I suffered from intermittent brain fog for years and also felt like my heart was maybe struggling more than usual when I was training at the gym.

Never enough to warrant going to a doctor unless I was being super paranoid (and spend a long time convincing them I wasn't paranoid) but just enough to always wonder if there was something more to the story.

ZpJuUuNaQ5•10m ago
Similar situation, but I had (and still have) issues with the heart, and not sure whether they were from undiagnosed covid infection (never had typical Covid symptoms, and all tests for it came negative), or some rare complications from the vaccine.

I started having heart flutters a day or two after my shot and had severe fever (I was 25 at the time), but the former never went a way. I brushed it off as a temporary symptom and typical after shot reaction (well, fever was at least). Heart flutters never went away and didn't go to the doctor for at least a few months, it became so frequent that I could not sleep, exercise or even climb the stairs anymore without heart feeling like it's about to explode out of my chest. It's not the high heart rate, but abrupt, irregular vibrations/twitching and sometimes feeling like you get punched in the chest, just from the inside around the heart area. Anyway, it happens frequently and in any situation, even at rest.

Got diagnosed with third degree AV block. The flutters were due to the failure of the conduction system - signals from the upper chambers did not always reach the lower chambers. I am not angry at anyone or anything, just disappointed. It would feel a lot easier if it was some obvious bad decision of mine, like drinking, doing drugs, smoking, or being overweight, but I did not nor were any of these things. I still keep categorizing life as "before" and "after".

tim333•33m ago
I haven't looked it up but often odd numbers like that are often due to the paper looking at people who were hospitalised for covid which is a small percentage of those who get it.
itsme0000•12m ago
Yeah the paper is just lying. “Long Covid” is just a typical response to a severe flu. Many people are just ignorant of the fact that flus can weaken the immune system past the initial infection. It’s well meaning attempt to understand common flu symptoms but they are just relating to COVID to get more clicks and funding.
ipsento606•1h ago
Edit - this comment is incorrect

---

The title should be edited. It sounds as if the test is 94% accurate at detecting long covid, but in fact it's 94% accurate at counting microclots

> We estimated a 94% accuracy for the microclot count using the devices, significantly higher than the traditional counting of microclots on slides (66% accuracy)

diffeomorphism•48m ago
No, you are parsing this wrong.

> We evaluated the diagnostic power (...). We estimated a 94% accuracy for (our method), significantly higher than the (traditional method) (66% accuracy).

Both methods have counting in their name, but they are comparing the diagnostic power.

ipsento606•44m ago
after reading more - you're right, I'm wrong

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Blood test detecting Long Covid in kids with 94% accuracy microclots

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